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ÀÌÁ¦ transfection¿¡ ´ëÇÑ °ÆÁ¤Àº ¾È Çϼŵµ µË´Ï´Ù. |
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»õ·Î¿î Transfection ±â¼ú !! |
ÀÌ ¹æ¹ýÀº ¾çÀÌ¿Â ¼º ºÐÀÚ¸¦ ÄÚÆÃÇÑ ÀÚ¼º ³ª³ëÀÔÀÚ¿Í DNA¿ÍÀÇ °áÇÕÀ» ÀÌ¿ëÇÑ °ÍÀÔ´Ï´Ù. MagnetofectionÀº À¯ÀüÀÚ º¤ÅÍ¿¡ ÀÚ·ÂÀ» ÁÖ¾î ¸ñÀûÇÏ´Â ¼¼Æ÷ ¾ÈÀ¸·Î µé¾î°¥ ¼ö ÀÖµµ·Ï ÇÕ´Ï´Ù. ÀÌ·¯ÇÑ ¹æ¹ýÀ¸·Î ¼ö ºÐ ¾È¿¡ ³Ö¾îÁØ ¸ðµç º¤ÅÍ°¡ ¼¼Æ÷ ¾ÈÀ¸·Î µé¾î°¡ 100%ÀÇ ¼¼Æ÷°¡ ÃæºÐÇÑ ¾çÀÇ º¤Å͸¦ Áö´Ï°Ô µË´Ï´Ù. ÀÔÁõµÇ°í ÀÎÁ¤ ¹ÞÀº ÀÚ¼ºÀ» ÀÌ¿ëÇÑ targeting±â¼úÀ» ÀÌ¿ëÇÑ ¹æ¹ýÀ¸·Î, ÀÌ ±â¼úÀº ´Ü¼øÇÏ°í, ºü¸£¸ç, ³ôÀº È¿À²À» Áö´Ï°í, ¹ü¿ëÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, ÀÀ¿ëÀÌ ´Ù¾çÇÏ°í, ¼¼Æ÷¿¡ ´ëÇØ ¹«µ¶Çϸç, ±×¸®°í °æÁ¦ÀûÀÎ ¹æ¹ýÀ̶ó°í ÇÒ ¼ö ÀÖ½À´Ï´Ù.
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´Ü¼øÇÑ ¹æ¹ý ±×¸®°í ½Å¼ÓÇÔ |
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°íÈ¿À² |
ÀÚ¼ºÀÔÀÚ¸¦ ¼¼Æ÷ ³»¿¡ ÁýÁß½ÃÅ´À¸·Î½á, ¾ÆÁÖ ÀûÀº ¾çÀÇ DNA ³ªvector·Îµµ transfection È¿À²À» ±Ø´ëÈ ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Confluent Primary Human Keratinocytes |
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Standard Transfection Commercial Reagent L |
Magnetofection (PolyMag) |
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¾î´À ¼¼Æ÷¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù. |
primary cells¿¡ transfection½ÃÅ°´Â ¹æ¹ý Áß °¡Àå ÀÌ»óÀûÀÎ ¹æ¹ýÀ̶ó°í ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Primary Cells Chondrocytes (Photo)
Peripherical Blood Lymphocytes
Aortic Endothelial Cells
Human Fibroblasts
Nasal & Pulmonary Epithelium... |
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¼¼Æ÷¿¡ transfection½ÃÅ°´Â ¹æ¹ý Áß¿¡¼ °¡Àå È¿À²ÀûÀÎ ¹æ¹ýÀÔ´Ï´Ù. |
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Cell Lines 293
MCF-7
HeLa
Hep G2
CHO-K1 |
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´Ù¾ç¼º |
OZ Biosciences´Â µÎ °¡Áö Á¾·ùÀÇ ½Ã¾àÀ» Á¦°øÇØ µå¸®°í ÀÖ½À´Ï´Ù. |
PolyMag
CT-26 Colon Carcinoma |
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Standard Transfection |
Magnetofection |
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PolyMag ´Â ³ôÀº È¿À²·Î ÇÙ»êÀ» ¼¼Æ÷ ³»¿¡ Àü´ÞÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. naked DNA, plasmid DNA, antisense oligonucleotides and siRNA µîÀÇ transfectionÀ» ¼º°øÀûÀ¸·Î µµ¿Í µå¸³´Ï´Ù. |
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NIH 3T3 Cells lacking CAR receptor infected with an Adenovirus (coding for LacZ) +/- CombiMag with (right) and without (left) magnetic field
CombiMag ´Â ¸ðµç Á¾·ùÀÇ º¤ÅÍ¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Â À¯ÀÏÇÑ ¹æ¹ýÀÔ´Ï´Ù. ´ÙÀ½°ú °°ÀÌ º¹ÇÕÀûÀ¸·Î »ç¿ëÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù
¾î¶² »ó¾÷ÀûÀ¸·Î ÀÌ¿ë °¡´ÉÇÑ transfection ½Ã¾àÀ̳ª ¸ðµç Á¾·ùÀÇ ÇÙ»ê°ú ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù. Áö±Ý±îÁö »ç¿ëÇϽôø ¹æ¹ýÀ» ¹Ù²Ü ÇÊ¿ä ¾øÀÌ ±× ¹æ¹ý¿¡ ÷°¡ÇÏ¿© transfectionÈ¿À²À» ³î¶ø°Ô Áõ°¡½Ãų ¼ö ÀÖ½À´Ï´Ù.
¹ÙÀÌ·¯½º¿Í ÇÔ²² »ç¿ëÇÏ¿© ¼÷ÁÖÀÇ ¹ÙÀÌ·¯½º vvector¿¡ ´ëÇÑ Ä£È¼ºÀ» ³ÐÈ÷°í(adenoviruses), ¹ÙÀÌ·¯½ºÀÇ °¨¿°È¿À² (retroviruses)À» °³¼±ÇÒ ¼ö ÀÖ½À´Ï´Ù.
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Standard Transfection |
Magnetofection (CombiMag)
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¼¼Æ÷¿¡°Ôµµ ¿¬±¸ÀÚ¿¡°Ôµµ ¸ðµÎ ¾È¶ôÇÔÀ» µå¸³´Ï´Ù. |
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¹«µ¶¼º |
´õ ÀÌ»ó ¼¼Æ÷¿¡°Ô Àü±âÀûÀÎ Ãæ°ÝÀ» ÁÙ ÇÊ¿ä°¡ ¾ø½À´Ï´Ù. |
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°æÁ¦¼º |
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°ªºñ½Ñ ÀåÄ¡°¡ ÇÊ¿ä ¾ø½À´Ï´Ù.
Àç·á¸¦ ¾Æ³¥ ¼ö ÀÖ½À´Ï´Ù.¾ÆÁÖ ÀûÀº ¾çÀÇ DNA·Îµµ ÃæºÐÇÕ´Ï´Ù.
ºñ¿ëÀ» Àý¾àÇÒ ¼ö ÀÖ½À´Ï´Ù: ½Ã¾à »ç¿ë·®ÀÌ Àû½À´Ï´Ù.
ºñ¿ë´ëºñ ÃÖ°íÀÇ È¿À²
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Catalog Number |
Product |
Volume(¥ìL) |
Size (# of Transf./¥ìg of DNA) |
# of Transf./96 well plates |
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KC-30296 |
Starting Kit©ö |
2 X 100 |
2 X 100 |
2 X 1000 |
KM-30200 |
Selection Kit©÷ |
2 X 100 |
2 X 100 |
2 X 1000 |
MF-10096 |
Magnetic Plate |
- |
- |
- |
PM-10100 |
PolyMag 100 |
100 |
100 |
1000 |
PM-10200 |
PolyMag 200 |
200 |
200 |
2000 |
PM-11000 |
PolyMag 1000 |
1000 |
1000 |
10000 |
CM-20100 |
CombiMag 100 |
100 |
100 |
1000 |
CM-20200 |
CombiMag 200 |
200 |
200 |
2000 |
CM-21000 |
CombiMag 1000 |
1000 |
1000 |
10000 |
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1. Contain : PM10100 + CM20100 + magnetic plate
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2. Contain : PM10100 + CM20100
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©¬-galactosidase Assay Kit |
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TransfectionµÈ ¼¼Æ÷¿¡¼ ©¬-galactosidase ÀÇ ¹ßÇö Á¤µµ¸¦ Á¤·®ÀûÀ¸·Î ÃøÁ¤ÇÒ ¼ö ÀÖ´Â Å°Æ®·Î¼, ¹Î°¨ÇÑ ±âÁúÀÎ, CPRG and ONPG¸¦ »ç¿ëÇÏ¿© ÃøÁ¤ÇÏ´Â Å°Æ®ÀÔ´Ï´Ù. |
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X-Gal Staining Kit |
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in situ ©¬-galactosidase ¿°»öÀ» ¹ÙÅÁÀ¸·Î transfection efficiency À» °áÁ¤ÇÏ´Â µ¥ »ç¿ëÇÏ´Â Ready-to-use assay system ÀÔ´Ï´Ù. |
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